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Last
updated February 4, 2005
Saturday, MAY 14
8:00am–11:00am
4102—Imaging
of the Developing Organism: Tools for the Developmental
Biologist
PAS Mini Course
Chair:
Colin K.L. Phoon, New York University School of Medicine,
New York, NY
Rapid advances in developmental genetics over the
past decade have led to the generation of myriad animal
models of abnormal development and the elucidation of many
genes involved in development. Phenotypic analysis has
traditionally been limited to histological or in vitro
techniques. Innovations in sophisticated imaging
modalities now allow investigators to see the results of
genetic manipulation in striking detail, including in vivo
imaging of the embryo, three-dimensional reconstruction of
embryonic structures and functional analysis of the
cardiovascular system. Such imaging tools will prove
invaluable in linking genomic processes with their
phenotypic manifestations. Multi-modality, non-redundant
imaging can help investigators answer key biological
questions. This state of the art mini course is designed
to provide investigators specializing in developmental
processes with an overview of several current innovative
imaging approaches for the study of the embryonic and
early postnatal organism and to stimulate collaboration as
well as advances in phenotypic analyses.
Target Audience: Scientists involved in basic
developmental biology research from various fields,
including cardiology, neurology, cell biology,
developmental biology (patterning, etc.) and genetics.
Introduction: What Can Advanced Imaging Do for the
Developmental Biologist?
Colin
K.L. Phoon, New York University School of Medicine, New
York, NY
In Vivo Ultrasound and MR Microimaging of Mouse Brain
Development
Daniel
H. Turnbull, Skirball Institute of Biomolecular Medicine,
New York University School of Medicine, New York, NY
Optical Projection Tomography: A New Approach for 3D
Microscopy and Gene Expression
James
Sharpe, MRC Human Genetics Unit, Western General Hospital,
Edinburgh, United Kingdom
Quantifying Developmental Dynamics Using DPIV
Jay
R. Hove, Genome Research Institute, University of
Cincinnati, Cincinnati, OH
Break
Dynamic Imaging of Fluid Forces and Heart Motions in
Developing Embryos
Mary
E. Dickinson, California Institute of Technology, Beckman
Institute, Pasadena, CA
Mapping Cardiac Excitation in Embryonic and Adult
Hearts
Gregory
E. Morley, New York University School of Medicine, New
York, NY
Panel Discussion and Question & Answer
11:45am–2:45pm
4501—Fish,
Worms and Flies
PAS Mini Course
Chair:
Edward R.B. McCabe, Mattel Children's Hospital at UCLA,
Los Angeles, CA
One of the most important lessons of the Human Genome
Project is how similar we are to the organisms that
surround us. The similarities between our biology and
theirs means that they truly are models from which we
learn more about ourselves and our diseases. In this mini
course, we will see how the fruit fly, Drosophila
melanogaster, can be used to identify drugs for human
diseases. We will learn how the nematode worm, Caenorhabditis
elegans, can be used to investigate signaling pathways
that are preserved from worms to humans and are critical
to committing undifferentiated cells to differentiate
correctly. The zebrafish, Danio rerio, provides us
with a vertebrate model for studying organ systems similar
to our own. The presenters will provide a general overview
of their organism and then an in-depth description of
their research.
Target Audience: Investigators involved with or
interested in learning about research involving model
organisms. Appeal will be the strengths of these
non-mammalian models for investigations ranging from
developmental biology to high-throughput drug screens.
Overview of Non-mammalian Model Organisms
Edward
R.B. McCabe, Mattel Children's Hospital at UCLA, Los
Angeles, CA
Flies: Identifying New Drugs for Human Diseases
Juan
Botas, Baylor College of Medicine, Houston, TX
Worms: Signal Transduction and Cellular
Differentiation
David
M. Eisenmann, University of Maryland, Baltimore, MD
Fish: Developmental Genetics of the Heart
Didier
Stainier, University of California, San Francisco, CA
Discussion
Sponsored jointly by the AAP Section on
Cardiology and the Pediatric Academic Societies
1:00pm–3:00pm
4652—Neonatal
Infectious Disease and Inflammation
PAS Original Science Abstracts -
Platform Session
1:00pm–3:00pm
4654—Pulmonary
Vascular Biology
PAS Original Science Abstracts -
Poster Symposium
3:15pm–5:15pm
4840—Animal
Models of Cardiac Diseases
PAS Original Science Abstracts -
Platform Session
3:15pm–5:15pm
4842—Fetal
Origins of Adult Disease
PAS Original Science Abstracts -
Poster Symposium
3:15pm–5:15pm
4843—Gastroenterology
PAS Original Science Abstracts -
Platform Session
3:15pm–5:15pm
4851—Pathogenesis
of Bronchopulmonary Dysplasia
PAS Original Science Abstracts -
Platform Session
3:15pm–5:15pm
4871—Cell
Biology Methods
PAS Educational Workshop
Leader:
Kathleen Sakamoto, Los Angeles, CA
Recent advances in molecular and cellular techniques
have provided new approaches to studying the role of gene
function in a variety of human diseases. It is critical
for Pediatricians and Pediatric subspecialists to
understand the basis and use of these emerging
technologies. This workshop will provide an overview of
new cell biology methods that are currently being used.
The topics include RNA interference, purification of stem
cells, microarray analysis and gene profiling and
approaches to flow cytometry. Upon completion of this
workshop, participants will be able to describe (a) design
and application of siRNA and shRNA, (b) approaches to stem
cell purification, (c) application of gene
profiling/expression arrays, and (d) uses of FACs
analysis.
Sunday, MAY 15
8:00am–10:00am
5104—Whole
Genome Investigation To Identify Susceptibility Genes
PAS Topic Symposium
Chairs:
Diana W. Bianchi, Tufts-New England Medical Center,
Boston, MA and Eric P. Hoffman, Children's National
Medical Center, Washington, DC
With the completion of the human genome, new tools
are now available to identify the genetic determinants for
complex pediatric disorders. This symposium will introduce
these new tools and discuss how they are being applied to
three critically important clinical issues in pediatrics.
First, the use of the HapMap and other recent advances in
whole genome association studies will be reviewed. Second,
the pursuit of susceptibility genes relevant to drug
responsiveness will be presented in the realm of pediatric
organ transplantation. Third, the identification of
susceptibility genes for reading disability will be
discussed.
Finally, the genetics of neurodevelopmental disorders
will be highlighted. The next layer of fundamental
understanding of complex disorders in pediatrics will
depend heavily on such strategies, and this symposium will
relay the matching high levels of excitement and rigor
with which these pursuits should go forth.
Whole Genome Association Studies for Complex Traits
and Diseases: Role of the HapMap and Other Recent Advances
Joel
N. Hirschhorn, Children's Hospital, Boston, MA
Genetic Contribution to Graft and Patient Outcomes
After Solid Organ Transplantation
Steven
A. Webber, University of Pittsburgh School of Medicine,
Pittsburgh, PA
Identifying Susceptibility Genes for Reading
Disability
Jeffrey
R. Gruen, Yale University School of Medicine, New Haven,
CT
The Genetics of Neurodevelopmental Disorders
Anthony
P. Monaco, University of Oxford, Oxford, England
8:00am–10:00am
5146—Neonatal
CNS Injury
PAS Original Science Abstracts -
Platform Session
8:00am–10:00am
5150—Vascular
Mediators in Persistent Pulmonary Hypertension
PAS Original Science Abstracts -
Platform Session
8:00am–11:00am
5239—Transgenic
and Gene Knockout Methods in Mice
PAS Educational Workshop
Leader:
Louis Muglia, St. Louis, MO; Co-leader: Scott Saunders
Genetic analyses in mice provide a powerful approach
to understanding fundamental areas of interest in
pediatrics. Transgenic and gene knockout model systems in
mice have advanced our understanding of developmental,
physiological and cognitive processes difficult to dissect
mechanistically in either lower organisms or humans. In
this workshop, a practical framework for the design and
production of genetically altered mice will be presented.
Discussion will begin with availability of resources for
DNA analysis and clone acquisition and progress through
consideration of construct production for pronuclear
injection or introduction into embryonic stem cells.
Limitations in interpretation of findings obtained in
conventional transgenic or knockout animals will be
reviewed, and methods to overcome these limitations with
conditional knockouts or inducible transgenes will be
presented.
2:00pm–4:00pm
5521—Regulation
of Alveolar Epithelial Repair—or, How Do We Put It All
Back Together Again
PAS Topic Symposium
Chairs:
Rita Ryan, State University of New York at Buffalo, Women
and Children's Hospital of Buffalo, Buffalo, NY; and Heber
Nielsen, Tufts University School of Medicine, Tufts-New
England Medical Center, Boston, MA
Regulation of alveolar epithelial repair after many
forms of lung injury remains incompletely understood. The
type II cell is an important source of growth factors and
there are autocrine and paracrine mediators that are
altered during the repair process. Type I cells are the
primary covering of the alveolar epithelium, and their
restoration is critical to recapitulate normal repair.
This symposium will focus on the fundamental mechanisms of
epithelial repair after injury and examine connections
with lung development. Finally, relevance to current
clinical disease will be discussed.
Target Audience: Physician and basic scientists
interested in how the alveolar epithelium repairs itself
after injury and the relationship of lung repair with lung
development.
Introduction
Rita
M. Ryan, State University of New York at Buffalo, Women
and Children's Hospital of Buffalo, Buffalo, NY
Heber C. Nielsen, Tufts New England Medical Center,
Boston, MA
Type II Cell Mitogens
Timothy
D. Le Cras, Cincinnati Children's Hospital Medical Center,
Cincinnati, OH
Type II Cell Proliferation During Lung Injury and
Repair
Michael
A. O'Reilly, University of Rochester, Rochester, NY
Type I Cells in Alveolar Repair
Susan
H. Guttentag, Children's Hospital of Philadelphia,
Philadelphia, PA
Apoptosis in Alveolar Epithelial Repair
Lin
L. Mantell, Institute for Medical Research at North
Shore-Long Island Jewish, New York University School of
Medicine, Manhasset, NY
Translating Alveolar Epithelial Repair Fundamentals
to the Bedside
John
S. Torday, Los Angeles Biomedical Research Institute at
Harbor-UCLA Medical Center, Torrance, CA
2:00pm–5:00pm
5595—Proteomic
Approaches to Diseases
SPR Special Interest Group
Chairs:
James Schilling, Department of Pediatrics, Stanford
University School of Medicine, Stanford, CA; and Anne
Murphy, Johns Hopkins University School of Medicine,
Baltimore, MD
Proteomics is a rapidly evolving discipline that has
the potential to provide insights into fundamental biology
of disease and deliver clinically useful biomarkers. This
session will focus on the application of different
proteomic technologies in diverse diseases and demonstrate
the impact proteomics can make.
Target Audience: Scientists and clinicians interested
in using newly emerging proteomics methods for the
elucidation of disease mechanisms and biomarker discovery.
Serum Proteomics and Biomarkers in Cardiovascular
Diseases
Jennifer
Van Eyk, Johns Hopkins School of Medicine, Bayview
Proteomics Center, Baltimore, MD
Application of Proteomics to Cystic Fibrosis
Harvey
B. Pollard, Uniform Health Services University, Bethesda,
MD
Autoimmune Diseases and Protein Arrays
P.J.
Utz, Stanford University Proteomics Center, Stanford, CA
Panel Discussion: Speakers and Session Chairs
Discussion
Sponsored by the Society for Pediatric
Research
4:15pm–5:45pm
5703—Stem
Cell Therapies: What's On the Horizon for Pediatrics and
Pediatric Diseases
PAS State of the Art Plenary
Chair:
Stuart Orkin, Dana Farber Cancer Institute, Boston, MA
Stem cell-based approaches hold great promise for
treating many of the tissue degenerative disorders that
afflict our aging population. This symposium will explore
the role of stem cell therapies in pediatric disorders due
to inborn errors of metabolism and other single-gene
defects. Furthermore, the symposium will discuss the
implications of new data indicating that fetal cells in
the maternal circulation can participate in maternal wound
repair, implying that the fetus may be able to “treat”
its mother.
Target Audience: Broad appeal for scientists and
clinicians interested in new therapeutic approaches based
on stem cell biology.
Stem Cell Therapy in Lysosomal Storage Disorders
Susan
L. Staba, University of Florida, Gainesville, FL
Liver Repopulation with Stem Cells
Markus
Grompe, Oregon Health & Science University, Portland,
OR
Pregnancy-Associated Stem Cells: Does the Fetus
"Treat" Its Mother?
Diana
W. Bianchi, Tufts University School of Medicine, Floating
Hospital for Children, Boston, MA
Summation and Perspectives
Stuart
H. Orkin, Dana Farber Cancer Institute, Boston, MA
4:15pm–6:15pm
5743—Pediatric
Nutrition and Metabolism
PAS Original Science Abstracts -
Platform Session
Monday, MAY 16
8:00am–10:00am
6131—Brain
Metabolism and Injury
PAS Original Science Abstracts -
Platform Session
8:00am–10:00am
6132—Clinical
Trials in Perinatal and Neonatal Medicine I
PAS Original Science Abstracts -
Platform Session
8:00am–10:00am
6133—Development
Biology
PAS Original Science Abstracts -
Platform Session
8:00am–10:00am
6137—Infectious
Diseases
PAS Original Science Abstracts -
Platform Session
8:00am–10:00am
6140—Neonatal–Fetal
Nutrition and Metabolism II
PAS Original Science Abstracts -
Poster Symposium
9:00am–12:00pm
6205—Developmental
and Molecular Imaging
PAS Educational Workshop
Leader:
Colin K.L. Phoon, New York, NY; Co-leader: Christopher
Contag
Advances in imaging now allow characterization of
many developmental processes, particularly in the in vivo
organism. This workshop is designed to introduce the
audience to two such techniques: ultrasound biomicroscopy
and biophotonic imaging, in detail. The emphasis is on
practical aspects of imaging, with the opportunity for
hands-on imaging and use of representative systems.
3:00pm–5:00pm
6730—Cardiac
and Pulmonary Development
PAS Original Science Abstracts -
Platform Session
3:00pm–5:00pm
6732—Neonatal
Hyperoxia and the Lung
PAS Original Science Abstracts -
Platform Session
3:00pm–5:00pm
6760—Functional
Genomics
PAS Educational Workshop
Leader:
Clifford W. Bogue, New Haven, CT; Co-leaders: James D.
Bristow and Cecilia Lo
Understanding the function of genes
and other parts of the genome is known as functional
genomics. The Human Genome Project is just the first step
in understanding humans at the molecular level. Now that
the sequencing phase of the human and mouse genomes is
complete, many questions remain unanswered, including the
function and regulation of most of the estimated
30,000-35,000 mouse and human genes. The mouse has a long
and rich history in biological research and many consider
it a model organism for the study of human development and
disease. Over the past few years, exciting progress has
been made in developing techniques for chromosome
engineering, mutagenesis, mapping and maintenance of
mutations and identification of mutant genes in the mouse.
Additionally, whole genome sequence analysis of many
different species is proving to be incredibly fruitful in
identifying critical gene regulatory motifs. In this
workshop, we will present some of the techniques that are
being applied to the daunting yet exciting task of
functional genomic analysis in the mouse.
Objectives:
- To learn about contemporary scientific techniques currently in use
to determine the function of the genome.
- To learn how the genomic sequence of various organisms can be used
to determine gene function.
Method of Instruction: Three lectures with
question-and-answer sessions.
Target Audience: Trainee, junior faculty, mid-level
faculty, senior faculty.
3:00pm–5:00pm
6762—Grant
Writing
PAS Educational Workshop
Leader:
William Hay, Denver, CO
The purpose of this workshop is to review the most
successful methods for writing a grant application. NIH
grant applications will be emphasized and used for
illustrations, but the format will be general enough to
apply to other granting agency applications. Specific
topics will include: career timeline for different grants,
organization of the application, items to include in each
section of the grant, budget requirements, writing hints,
resubmission strategies, how to write the application to
meet study section reviewer criteria, common successes and
common mistakes in writing applications. A handout of the
Powerpoint figures used in Dr. Hay's talk will be
available. There will be ample time for general and
personal questions from the attendees.
3:00pm–5:00pm
6764—NICHD:
How It Works and Opportunities for Research Support
PAS Educational Workshop
Leader:
Duane Alexander, Bethesda, MD; Co-leader: Linda Wright
This grantsmanship session will describe the process
of applying for research support from the NIH, how review
is done and funding decisions made and how to enhance the
likelihood of success. Mechanisms available for support at
various stages of a career will be reviewed, as well as
current research topics of special interest to NICHD.
Objective:
-
Areas of special current research interest to NICHD.
- Information
on a variety of support mechanisms at different career stages and
how NIH receives, assigns, reviews and funds
applications for support of various types of research,
training and career development.
Method of Instruction: Presentations followed by
questions and answer.
Target Audience: Beginner.
3:00pm–5:00pm
6765—Translational
Proteomics in Pediatric Investigations
PAS Educational Workshop
Leader:
Asher D. Schachter, Boston, MA; Co-leader: Emanuel F.
Petricoin
This workshop will provide an introduction and
overview of cutting-edge techniques in proteomics, as
applied to identifying non-invasive diagnostic and
prognostic disease biomarkers. Workshop participants will
(1) learn the basics of how this technology works, (2) see
actual data from translational proteomics analysis of
serum and urine, (3) learn the fundamentals of the
analytical and informatic methods commonly used to
interpret the data and (4) have the opportunity to ask
questions and discuss certain topics of interest, such as
pitfalls and limitations of these techniques. Upon
completion of the workshop, participants will have a
fundamental knowledge of translational proteomics.
Objectives:
- Attain knowledge of the fundamentals of proteomics.
- Understand the fundamentals of identifying biomarkers for clinical
applications.
Method of Instruction: An interactive presentation
followed by a question-and-answer period.
Target Audience: Trainee, junior faculty, mid-level
faculty.
3:00pm–5:00pm
6766—Writing
a Scientific Paper
PAS Educational Workshop
Leader:
Kurt Albertine, Salt Lake City, UT
The goal of this workshop is to provide practical
guidance for composing a manuscript. Emphasis is placed on
planning, organizing and composing a clear scientific
document. All parts of a manuscript will be discussed
(Abstract, Introduction, Methods, Results, Discussion,
References, Figures, Tables). Other topics will be journal
selection, authorship and the review process. Questions to
be addressed will include:
- What is the question asked, or hypothesis tested, by your study?
- Is the question or hypothesis stated clearly and explicitly?
- Are the methods suitable?
- Do the results (data) answer the question or address the
hypothesis?
- Are the figures and tables clear?
- Are the figures and tables redundant?
- Is information included that is irrelevant to the purpose of the
study?
- Are the statistics suitable?
- Are the references adequate?
- Does the discussion contain smokescreens, straw men or omit
alternate theories or contrary evidence?
- Are limitations to the study discussed?
- What is the importance or significance of your study?
- Is the manuscript easy to read and prepared carefully?
Those planning to attend this workshop should obtain
the following two (2) books, both of which are paperback.
-
Zeiger M. Essentials of Writing Biomedical Research
Papers. 2nd edition, McGraw Hill, Inc., NY, 2000.
(ISBN 0-07-072833-X). (~$45)
- Briscoe MH. Preparing Scientific Illustrations. 2nd edition,
Springer, NY, 1996. (ISBN 0-387-94581-4). (~$30)
Also, please bring a pad of writing paper and
pencils/pens. Participants may bring a manuscript that
they are currently composing. Please do not bring a
published paper because published manuscripts cannot be
revised.
A textbook reference list will be provided to
participants
Tuesday, MAY 17
8:00am–10:00am
7157—Lung
Maturation, Septation and Growth
PAS Original Science Abstracts -
Platform Session
8:00am–10:00am
7158—Mechanisms
of Childhood Lung Disease
PAS Original Science Abstracts -
Platform Session
8:00am–10:00am
7159—Neonatal
Neurology—Experimental Models
PAS Original Science Abstracts -
Poster Symposium
10:15am–11:45am
7300—Children's
Health and the Federal Government: Research and Public
Health Policy
PAS State of the Art Plenary
Chairs:
Lisa Guay-Woodford, President, Society for Pediatric
Research; and Paul Young, Chair, PAS Program Committee
Elias A. Zerhouni, the Director of the NIH and Vice
Admiral Richard H. Carmona, the Surgeon General of the
United States, will provide PAS attendees with their views
of the critical issues related to pediatric research and
the health of our nation's children.
Target Audience: All attendees
Introduction
Paul
C. Young, University of Utah School of Medicine, Salt Lake
City, UT
Lisa M. Guay-Woodford, University of Alabama at
Birmingham, Birmingham, AL
The NIH Roadmap for Medical Research
Elias
A. Zerhouni, Director, National Institutes of Health,
Bethesda, MD
Promoting Health for U.S. Children and Their Families
Vice
Admiral Richard H. Carmona, Surgeon General of the United
States, Washington, DC
Discussion
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